| Correlate™-EIA (ELISA Kits) |
|
 |
| human
Gastrin I Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-026 |
|
|
|
| 5 x 96 Well Kit |
901-026 |
|
|
|
| rat
Gastrin I Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-149 |
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Time to Answer: 5 Hours
Easy To Use
Small Sample Volumes: 50 & 100 µL
Human Gastrin I (1-17) Sequence:
H2N-Glu-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2
Rat Gastrin I (G17) Sequence:
Pyr-Arg-Pro-Pro-Met-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2
|
Gastrins are a family of sequence-related carboxyamidated peptides
produced by endocrine G cells of the antrum mucosa in response to a
number of stimuli associated with digestion. Antral distension,
partially digested proteins, amino acids and vagal stimulation all
contribute to gastrin release from G cell storage. Increased serum
gastrin levels are associated with duodenal ulcers, Helicobacter
pylori infections, colorectal carcinomas and other tumors and
cancerous lesions. Gastrin is the most potent stimulator of gastric acid
secretion.
Gastrin is synthesized as a 101 residue pre-pro-peptide on the rough
endoplasmic reticulum, then post-translationally modified by cleavage
and alpha-amidation to result in the active forms G34, G17 and G13/14.
There are two types of G17 and G34: type II is sulfated at the tyrosine
reside, while type I is not. G34 is the major circulating gastrin in
fasting serum, but after feeding, G17 levels increase two to three-fold
until both are present in approximately equal amounts. |
|
|
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
|
| |
human Gastrin
(900-026 & 901-026) |
rat Gastrin
(900-149) |
| RANGE |
39.1-10,000 pg/mL
|
78.1 - 5,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
50 µL |
| SAMPLES PER 96
WELL KIT |
39 in duplicate
|
37 in duplicate
|
| SENSITIVITY |
7.27 pg/mL
|
78.1 pg/mL
|
| 50% INTERCEPT |
282 pg/mL
|
269 pg/mL
|
| PRECISION |
3.7-8.8% Intra
3.4-6.5% Inter |
8.3-8.8% Intra
7.0-14.7% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
serum ( 1:8)
citrate plasma (1:8)
|
culture media (none)
serum (1:16)
EDTA plasma (1:16)
|
| TIME TO ANSWER |
5 Hours |
5 Hours |
| SPECIES
SPECIFICITY |
human |
rat |
|
| human |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| Gastrin I (1-17) |
100% |
Minigastrin (G13-I) |
74.6% |
| rat Gastrin I |
70.7% |
Gastrin II (G-17-II, sulfated) |
9.30% |
| Cholecystokinin 26-33 (CCK-8) |
2.67% |
Gastrin Tetrapeptide (CCK-4) |
1.60% |
| Big Gastrin (G34-I) |
0.80% |
--- |
--- |
| < 0.001%: Gastrin Releasing
Peptide (GRP) / Gastrin Inhibitory Polypeptide (GIP) / Glucagon
/ Bombesin / Pancreatic Polypeptide / Vasoactive Intestinal
Peptide (VIP) / Somatostatin-14 |
| rat |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| rat Gastrin I |
100% |
Cholesystokinin 26-33 (CCK-8) |
100% |
| human Gastrin I |
30% |
mouse Gastrin I |
20.5% |
| Minigastrin (G13-I) |
11% |
Gastrin Tetrapeptide (CCK-4) |
0.75% |
| Glucagon |
0.5% |
--- |
--- |
< 0.2%: Gastrin Inhibitory
Polypeptide (GIP) / Bombesin / Somatostatin-14 / Gastrin
Releasing Peptide (GRP)
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45