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Flashlight Acridinium
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Flashlight® Reagents |
| Product |
Catalog # |
Spec Sheet |
| Flashlight®-Streptavidin |
906-021 |
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| Flashlight®-Biotin |
906-022 |
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| Flashlight®-GxM IgG |
906-023 |
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| Flashlight®-GxR IgG |
906-024 |
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| Flashlight®-Protein A |
906-025 |
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| Flashlight®-Protein G |
906-026 |
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| Flashlight®-Trigger Solution |
906-020 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
Direct Chemiluminescent Label
Detect less than 1 attomole
Stable to High Temperatures & pH
Labeled Proteins Stable at 4 degrees Celcius
Single Trigger System
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Chemiluminescence is the emission of light from a chemical reaction that
occurs at or near ambient temperatures. A vast number of reactions give
rise to the emission of light in solution, but only a few have
sufficiently high efficiencies of chemiluminescence to have been used
for analytical purposes. For further information on the vast array of
chemical reactions that produce light please refer to excellent reviews
by McCapra and Beheshti in Bioluminescence and Chemiluminescence:
Instruments and Applications, 1985, Ed. K. Van Dyke, CRC Press, Boca
Raton, FL. pgs. 9-42; and by Schuster and Smith,Adv. Phys. Org. Chem.,
1982, 18, 187.
Acridinium esters have moderate chemiluminescence efficiencies and
the chemistry of light production is well understood.They have the added
benefit that they require simple triggering conditions that contribute
little to the background signal.This benefit has been exploited to great
effect in high sensitivity immunoassay applications.These compounds can
also be easily modified to attach to proteins and other molecules of
interest, without affecting their chemiluminescence properties
significantly.
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The Flashlight® labeling system is ideally suited for
applications requiring high sensitivity, such as high sensitivity
immunoassays for thyroid peptide hormones, free hormone fractions,
steroids, and drugs. It can also be used for labeling DNA or RNA probes.
Universal labeling systems, such as avidin, streptavidin, antibodies to
fluorescein, antibodies to DNA complexes, etc. are also easily made. We
offer a range of Flashlight® labeled materials. These
materials are supplied in buffered saline, containing protein
stabilizers and detergent. Gentamicin has been added as a preservative.
The Flashlight® labeled materials may be used in any
buffer system, phosphate buffered saline, Tris buffered saline, or Hepes
buffered saline, for example. Incubations at elevated temperatures
(>45ˇC) and at high pH's (>8.0) should be kept to a minimum. In general,
Flashlight® labeled materials are most stable in the pH range
5 to 7, and at or below room temperature. Storage at dilute
concentrations should be carried out in buffers containing detergents
(Triton X-100, Tween 20, etc.) and protein carriers (BSA, HSA, etc.).
After the binding reaction, the bound Flashlight™ labeled material,
or the supernatant containing unbound Flashlight® labeled
material may be analyzed for chemiluminescence. In analyzing the bound
Flashlight® labeled material, no pretreatment is necessary.
The solid phase with the bound Flashlight® labeled material
is placed into a suitable luminometer, and the Trigger Solution (Assay
Designs Catalog No. 90620) added. Maximum light emission occurs at 420
to 430 nm and is compatible with all commercially available
luminometers. The emission starts as soon as the trigger is added and
lasts for about 2 seconds. |
The kinetics of light emission generated by the Flashlight®
label is shown below. The actual kinetics obtained will depend upon the
luminometer injection system and the volume of Trigger Solution used.
This curve was obtained on a Berthold Clinilumat luminometer with 300 µl
injection of the Trigger Solution, and a 1.2 second injection time.
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The stability of the Flashlight® label is shown on the graphs
below. Data is given for the long term stability of Flashlight®
labeled material at 4 degrees Celcius, and at elevated temperatures.
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| Assay Designs sells a wide range of Flashlight® Labeled
Antibodies, Proteins and haptens for use in a variety of detection
systems. These are listed below. Please contact us for new Flashlight®
labeled materials and for Custom labeling information. |
| Material |
Catalog Number |
Quantity |
No. of
Determinations |
| Flashlight®-Streptavidin |
906-021
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50 µg |
>1,000 |
| Flashlight®-Biotin |
906-022
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50 µg |
>1,000 |
| Flashlight®-Goat anti-Mouse IgG,
Fc |
906-023
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50 µg |
>1,000 |
| Flashlight®-Goat anti-Rabbit
IgG, Fc |
906-024
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50 µg |
>1,000 |
| Flashlight®-Protein A |
906-025
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50 µg |
>1,000 |
| Flashlight®-Protein G |
906-026
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50 µg |
>1,000 |
| Flashlight®-Trigger Solution
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906-020
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250 mL |
>800 |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45