| TiterZyme® Kits
(ELISA Kits) |
|
 |
|
Endothelin-1 Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human: 96 Well Kit |
900-020 |
|
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|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Sandwich EIA Procedure
2 Convenient Formats
Time to Answer: 2 Hours or Overnight
|
Endothelins (ET) are isopeptides produced by vascular endothelium that
have potent vasoconstriction activity. The peptides are encoded by three
separate genes and processed to yield 39-residue
"Big ET" molecules which are further processed to the 21 amino acid
sequences designated ET-1, ET-2 and
ET-3. Vascular endothelial injury stimulates the release of
endothelins, which activate macrophages and act as strong
chemo-attractants for circulating monocytes. Endothelins affect vascular
smooth muscle fibroblast proliferation; help regulate vascular, airway,
and intestinal smooth muscle tone; increase the activity of bone
alkaline phosphatase; stimulate release of atrial natriuretic peptide
(ANP) from atrial cardiocytes; inhibit the release of renin from
glomeruli and modulate norepinephrine at sympathetic nerve termini.
Opposing vasomotor effects are regulated through the binding of distinct
endothelin receptors for vasoconstriction and vasodilation. Endothelins
have been linked to atherosclerosis and various cardiovascular disease
states. ET-1 is important in congestive heart failure, renal failure,
pulmonary hypertension, hyperlipidemia and metastatic prostate cancer.
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|
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1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Substrate into the wells and incubate.
6. Pipet Stop Solution and read on a plate reader at 450 nm.
7. Calculate sample concentrations from Standard Curve.
|
| |
human ET-1
(900-020) |
| RANGE |
0.78-100 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
38 in duplicate
|
| SENSITIVITY |
0.14 pg/mL
|
| PRECISION |
4.6-8.5% Intra
3.1-8.1% Inter |
SAMPLE TYPE
recommended dilution |
culture media ( 1:2)
serum (1:32)
EDTA plasma (1:8)
|
| TIME TO ANSWER |
2 Hours |
| SPECIES
SPECIFICITY |
human |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| Endothelin-1 (1-21) |
100% |
|
|
| < 0.10%: Endothelin-2
(1-21) / Endothelin-1 (1-31) / rat Big Endothelin-1 /
Endothelin-3 (1-21) / Endothelin-2 (1-31) / Endothelin-3 (1-31)
/ Big Endothelin-1 / Big Endothelin-2 / Big Endothelin-3 / VIC
(mouse ET-2) |
| Human Endothelin-1 has identical amino acid
sequence to bovine, canine, murine, porcine, rabbit and rat
Endothelin-1. |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45