FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Low Cross Reactivity to Other Steroids
All Steps at Room Temperature
Stable, Color-Coded Liquid Reagents
Time to Answer: 3 Hours
Estriol is a female sex steroid hormone produced by the placenta during
pregnancy and is the major estrogen produced in the normal fetus. In
non-pregnant circumstances, estriol is derived almost exclusively from
17beta-Estradiol. Estriol exists in biological matrix in
unconjugated (~9%) and conjugated forms (~91%). Conjugation with
glucuronic or sulfuric acid of estriol occurs in the liver, which aides
in steroid solubility, therefore allowing rapid elimination via the
kidney.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:16)