FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Low Cross Reactivity to Other Steroids
All Steps at Room Temperature
Time to Answer: 5 Hours
Dehydroepiandrosterone (DHEA) is a 17-ketosteroid produced by side chain
cleavage of 17-hydroxypregnenolone. DHEA is reversibly converted to its
sulfate ester (DHEA-S) and Androstenediol. DHEA and DHEA-S are the major
secretory products of the human adrenal glands and are precursors for
both estrogenic and androgenic steroids. DHEA has been linked to a
number of physiological conditions inlcuding: Depression, Systemic Lupus
Erythematosus and Rheumatoid Arthritis.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:8)