FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Measures LTC4, LTD4 and LTE4
Small Sample Volume: 100 µL
Time to Answer: 4 Hours
The Cysteinyl Leukotrienes (LTC4, LTD4, and LTE4)
are metabolites of arachidonic acid. They are formed via the
5-lipoxygenase pathway by addition of cysteine derivatives to LTA4.
The conversion of LTA4 to LTC4 by the enzyme LTC4
synthase limits the rate of cysteinyl leukotriene formation. The
cysteinyl leukotrienes are potent lipid mediators in inflammation. They
cause smooth muscle contractions and increased capillary permeability.
Studies have also shown that cysteinyl leukotrienes has been useful in
several studies, such as Hepatitis B, Helicobacter pylori infections,
cerebral vasospasms and blood-brain cell contact, as well as asthma and
other inflammatory responses.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. EIA: Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
