| Correlate™-EIA, FPIA (ELISA
Kits) |
|
 |
| Cortisol
Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
900-071 |
|
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|
| 5 x 96 Well Kit |
901-071 |
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|
Cortisol Fluorescence Polarization Immunoassay (FPIA)
Kits |
| Description |
Catalog #
|
Kit Insert
|
MSDS |
A homogeneous, quantitative
method of measuring Cortisol in cell supernatants. The
kit includes bulk packaged reagents for 100 tests of 100
µL volume.
|
920-071 |
|
|
|
|
|
|
| |
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
| |
Small Sample Volume: 100 µL
EIA Time to Answer: 3 Hours
Stable, Color-Coded Liquid Reagents
All Steps at Room Temperature
|
Cortisol (hydrocortisone, compound F) is a steroid hormone synthesized
from cholesterol. Cortisol is the primary glucocorticoid produced and
secreted by the adrenal cortex. Cortisol is found in the blood as either
free cortisol, or more commonly, bound to corticosteroid-binding
globulin (CBG). Serum levels are highest in the early morning and
decrease throughout the day. Cortisol is involved primarily in metabolic
and immunological actions. In the metabolic aspect, cortisol promotes
gluconeogenesis, liver glycogen deposition, and the reduction of glucose
utilization. Immunologically, cortisol functions as an important
anti-inflammatory and cortisol plays a role in hypersensitivity,
immunosuppression and disease resistance. Factors such as age, various
diseases, nutrition and other hormone levels affect cortisol secretion.
It has also been shown that plasma cortisol levels elevate in response
to stress. Abnormal cortisol levels are being tested for correlation
with a variety of different conditions, including prostate cancer,
depression and schizophrenia, while it is already known that an excess
of cortisol in all bodily tissues is the cause of Cushings Syndrome.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
|
| |
Cortisol EIA
(900-071 & 901-071) |
Cortisol FPIA
(920-071) |
| RANGE |
156-10,000 pg/mL
|
781-800,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
100 µL/96 well
40 µL/384 well |
| SAMPLES PER 96
WELL KIT |
37 in duplicate
|
100 tests at
100 µL |
| SENSITIVITY |
56.72 pg/mL
|
685 pg/mL
|
| 50% INTERCEPT |
957 pg/mL
|
|
| Z-FACTOR |
n/a |
0.823 |
| PRECISION |
6.6-10.5% Intra
7.8-13.4% Inter |
20-27% Intra
18-26% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
saliva ( 1:4)
urine (none)
serum (1:8)
plasma (1:8)
|
cell supernatants
|
| TIME TO ANSWER |
3 Hours |
minimum of 30 minutes
|
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
|
| EIA |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| Cortisol |
100% |
Prednisolone |
122.35% |
| Corticosterone |
27.68% |
11-deoxycortisol |
4.0% |
| Progesterone |
3.64% |
Prednisone |
0.85% |
| Testosterone |
0.12% |
Androstenedione |
<0.10% |
| Cortisone |
<0.10% |
Estradiol |
<0.10% |
| FPIA |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| 11-Deoxycortisol |
14% |
Prednisolone |
13% |
| Cortisone |
9.1% |
17alpha-Hydroxyprogesterone |
2.5% |
| 17-Hydroxypregnenolone |
2.5% |
Corticosterone |
2.0% |
| Progesterone |
1.1% |
Androstenedione |
0.5% |
| Prednisone |
0.5% |
17beta-Estradiol |
<0.1% |
| Testosterone |
<0.1% |
|
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|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45