FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Color-Coded, Stable, Liquid Reagents
Time to Answer: 3 Hours
Low Cross Reactivity to Other Steroids
Corticosterone (also referred to as Kendall's Compound B and Reichstein
Substance B) is a glucocorticoid secreted by the cortex of the adrenal
gland. Corticosterone is produced in response to the stimulation of the
adrenal cortex by adrenocorticotropic hormone (ACTH) and is the
precursor of aldosterone. Corticosterone is a major indicator of stress
since stress increases the production of corticosteroids. Studies
involving corticosterone and levels of stress include impairment of long
term memory retrieval, chronic corticosterone elevation due to dietary
restrictions and in response to burn injuries. In addition to stress
levels, corticosterone is believed to play a decisive role in sleep-wake
patterns.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Conjugate followed by the yellow Antibody into the
wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
Corticosterone
(900-097 & 901-097)
RANGE
32-20,000 pg/mL
SAMPLE SIZE
100 µL
SAMPLES PER 96
WELL KIT
39 in duplicate
SENSITIVITY
27.0 pg/mL
50% INTERCEPT
760 pg/mL
PRECISION
6.6-8.4% Intra
7.8-13.1% Inter
SAMPLE TYPE
recommended dilution
culture media (none)
saliva (1:16)
serum (1:8)
hep plasma (1:50)
TIME TO ANSWER
3 Hours
SPECIES
SPECIFICITY
non-specific
Compound
Cross Reactivity
Compound
Cross Reactivity
Corticosterone
100%
Deoxycorticosterone
21.3%
Desoxycorticosterone
21.0%
Progesterone
0.46%
Testosterone
0.31%
Tetrahydrocorticosterone
0.28%
Aldosterone
0.18%
Cortisol
0.046%
Pregnenolone
< 0.03%
beta Estradiol
< 0.03%
Cortisone
< 0.03%
11-dehydrocorticosterone acetate
< 0.03%
For more information :: Bioxys and Gentaur
BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:16)