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Chemiluminescent HTS Molecules
| Chemiluminescent Molecules |
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Luminescent Products |
| Product |
Catalog # |
Spec Sheet |
MSDS |
| MouseLightTM-Acridinium Ester
Labeled GxM IgG, 100 µL |
906-011 |
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| RabbitLightTM-Acridinium Ester
Labeled GxR IgG, 100 µL |
906-012 |
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| StreptLightTM-Acridinium Ester
Labeled Streptavidin, 50 µg |
906-013 |
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| BioLightTM-Acridinium Ester
Labeled Biotin, 50 µg |
906-014 |
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| Acridinium Ester Trigger Solution, 250 mL |
906-001 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
Rapid, Simple Signal Generation
Stable Reagents
Measure Attomoles of IgG's
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Assay Designs introduces our new line of Acridinium Ester-labeled
molecules for use as High Throughput Screening (HTS) detection systems.
We now supply affinity purified goat antibodies to rabbit and mouse Fc
molecules pre-labeled with chemiluminescent Acridinium Ester. Also
available is Acridinium Ester-Labeled Streptavidin for the detection of
biotin labeled probes and biomolecules, and Acridinium Ester-Labeled
Biotin for the detection of avidin and streptavidin labeled molecules.
Acridinium Ester systems are ideally suited for applications requiring
high sensitivity, such as highly sensitivity immunoassays for thyroid
peptide hormones, free hormone fractions, steroids, and drugs.
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Data depicting the detection of Acridinium-labeled mouse IgG, rabbit
IgG and biotinylated BSA are presented here. Using our standard
procedures, we were able to detect 1 pg of mouse IgG or 6.7 attomoles of
mouse IgG using MouseLightª-Acridinium Ester-labeled goat anti-mouse IgG
molecule.
Rabbit IgG was added to goat anti-rabbit IgG-coated plastic tubes. After
a short incubation the tubes were washed and RabbitLightª-Acridinium
Ester Labeled Goat anti-Rabbit IgG was added to the tubes. The tubes
were incubated, washed and then read for 2 seconds each after injection
of trigger solutions. Below are the graphs for the detection of rabbit
IgG molecules and biotinylated BSA.
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These chemiluminescent labeled materials may be used in any buffer
system, phosphate buffered saline, Tris buffered saline, or HEPES
buffered saline, for example. Incubations at elevated temperatures
(>45°C) and at high pHÕs (>8.0) should be minimized. In general, labeled
materials are most stable in the pH range 5 to 7, and at or below room
temperature. Storage at dilute concentrations should be carried out in
buffers containing detergents (Triton X-100, Tween 20, etc.) and protein
carriers (BSA, HSA, etc.). After the binding reaction, the bound labeled
material or the supernatant containing unbound labeled material may be
analyzed for chemiluminescence. When analyzing bound labeled material,
no pretreatment is necessary. The solid phase with the bound labeled
material is placed into a suitable luminometer and the chemiluminescent
reaction is triggered using either the Acridinium Ester Trigger
Solutions (Assay Designs Catalog No. 906-001) for the Acridinium
Ester-labeled materials. Maximum light emission occurs at 420 to 430 nm
and is compatible with all commercially available injecting luminometers.
The emission starts as soon as the trigger is added and lasts for about
2 seconds.
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