FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Stable, Easy to Use Reagents
Kinetic or Stopped Reaction
Caspase-6 is also known as Mch2. It is an intracellular cysteine
protease that exists as a proenzyme, becoming activated during the
sequence of events associated with apoptosis. Caspase-6 cleaves a
variety of cellular molecules that contain the amino acid theme VEID.
Apoptosis was originally reported in 1972 and was described as a
mechanism of controlled or programmed cell death. This process is very
common in bone marrow and organs with high proliferative activity. It
has also been implicated in the progression of a number of diseases,
including AIDS, cancer and autoimmune pathologies and has been
extensively studied by cellular biologists in fas-mediated cell death.
Apoptosis is characterized by a variety of changes including loss of
cellular membrane phospholipid symmetry, chromatin condensation,
mitochondrial swelling and eventually leads to damage and fragmentation
of DNA. This process results in cell death distinctly different from
necrosis. As a result, apoptotic cells avoid the inflammatory response
normally associated with necrosis.
1. Pipet Standards, Samples and Calibrator in duplicate into the wells.
2. Pipet Substrate into the wells and incubate.
3. Pipet Stop Solution (if desired).
4. Fluorometric: Read on a Fluorometer at 440 nm or with excitation at
360 nm.
5. Calculate sample concentrations from Standard Curve.
Fluorometric
Caspase-6 (907-021)
RANGE
2.39-57.1 Units/mL
SAMPLE SIZE
50 µL
SAMPLES PER 96
WELL KIT
40 in duplicate
SENSITIVITY
0.297 Units/mL
PRECISION
2.6-6.0% Intra
5.7-7.5% Inter
SPECIES
SPECIFICITY
non-specific
For more information :: Bioxys and Gentaur
BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
