FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Time to Answer: 3 Hours
Small Sample Volume: 100µL
The human Complement C4a des Arg TiterZyme® Enzyme Immunoassay
Kit is used for the quantitative determination of levels of the human
anaphylatoxin C4a in biological samples. The blood complement system has
a variety of defense mechanisms against microbial and viral infections.
Complement C4a is a peptide derived from the glycoprotein C4 during
activation of the complement system by the classical or the alternative
pathway. The C4a anaphylatoxin is very short-lived and in serum is
cleaved into the stable C4a des Arg. Quantitation of the des Arg
metabolite allows reliable conclusions about complement activation in
test samples. Activation of the complement system occurs during sepsis,
respiratory distress syndrome, multi-organ failure and in transplant
organ rejection.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
C4a des Arg
(900-059 & 901-059)
RANGE
0.78-200 ng/mL
SAMPLE SIZE
100 µL
SAMPLES PER 96
WELL KIT
39 in duplicate
SENSITIVITY
0.76 ng/mL
50% INTERCEPT
9.65 ng/mL
PRECISION
6.7-13.9% Intra
11.3-12.5% Inter
SAMPLE TYPE
recommended dilution
plasma (1:200)*
*when special sample handling instructions are followed
TIME TO ANSWER
3 Hours
SPECIES
SPECIFICITY
human
Compound
Cross Reactivity
Compound
Cross Reactivity
C4a des Arg
100%
C4
2.14%
C5a des Arg
0.28%
C3
0.23%
C3a des Arg
0.04%
C5
0.02%
For more information :: Bioxys and Gentaur
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