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Big ET-1
| TiterZyme® Kits
(ELISA Kits) |
|
 |
| Big
Endothelin-1 Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human: 96 Well Kit |
900-022 |
|
|
|
| rat: 96 Well Kit |
900-073 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Non-Radioactive
Time to Answer: Overnight Incubation
|
Endothelins (ET) are isopeptides produced by vascular endothelium that
have potent vasoconstriction activity. The peptides are encoded by three
separate genes and processed to yield 39-residue "Big ET" molecules
which are further processed to the 21 amino acid sequences designated
ET-1, ET-2 and
ET-3. Vascular endothelial injury stimulates the release of
endothelins, which activate macrophages and act as strong
chemo-attractants for circulating monocytes. Endothelins affect vascular
smooth muscle fibroblast proliferation; help regulate vascular, airway,
and intestinal smooth muscle tone; increase the activity of bone
alkaline phosphatase; stimulate release of atrial natriuretic peptide
(ANP) from atrial cardiocytes; inhibit the release of renin from
glomeruli and modulate norepinephrine at sympathetic nerve termini.
Opposing vasomotor effects are regulated through the binding of distinct
ET receptors for vasoconstriction and vasodilation. Endothelins have
been linked to atherosclerosis and various cardiovascular disease states.
ET-1 is important in congestive heart failure, renal failure,
pulmonary hypertension, hyperlipidemia and metastatic prostate cancer.
Big ET-1 is more stable than
ET-1 in plasma and may be useful in assessing ECE inhibitors. It
exerts similar effects in vivo, but has reduced vasoconstrictor
activity in vitro.
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1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Substrate into the wells and incubate.
6. Pipet Stop Solution and read on a plate reader at 450 nm.
7. Calculate sample concentrations from Standard Curve.
|
| |
human Big ET-1
(900-022) |
rat Big ET-1
(900-073) |
| RANGE |
0.78-100 pg/mL
|
0.78-100 pg/mL
|
| SAMPLE SIZE |
100 µL |
100 µL |
| SAMPLES PER 96
WELL KIT |
38 in duplicate
|
38 in duplicate
|
| SENSITIVITY |
0.23 pg/mL
|
0.72 pg/mL
|
| PRECISION |
4.9-12.8% Intra
1.9-4.6% Inter |
2.1-14.1% Intra
2.1-5.1% Inter |
SAMPLE TYPE
recommended dilution |
culture media (1:4)
serum ( 1:4)
|
culture media (1:2)
serum (1:16)
EDTA plasma (1:12)
|
| TIME TO ANSWER |
Overnight
|
Overnight
|
| SPECIES
SPECIFICITY |
human |
rat |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
|
human |
| Big Endothelin-1 |
100% |
rat Big Endothelin-1 |
100% |
Endothelin-1
(1-31) |
57.6% |
|
|
| <0.10%: human Big
Endothelin-2, human Big Endothelin-3, Endothelin-1 (1-21),
Endothelin-2 (1-21), Endothelin-3 (1-21), Endothelin-2 (1-31),
Endothelin-3 (1-31), VIC |
| |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
|
rat |
| rat Big Endothelin-1 |
100% |
human Big Endothelin-1 |
100% |
| <0.10%: rat Big
Endothelin-2, rat Big Endothelin-3, Endothelin-1, Endothelin-2,
Endothelin-3, VIC (mouse ET-2) |
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45