| TiterZyme® Kits
(ELISA Kits) |
|
 |
| human
total Bcl-2 TiterZyme® (EIA) Kit |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human total: 96 Well Kit |
900-133 |
|
|
|
|
|
| |
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Stable, Liquid Color-Coded Reagents
Time to Answer: 3 Hours
|
Bcl-2 (B-cell lymphoma 2) is an oncogenic protein that inhibits
apoptosis. It is located within the mitochondrial membrane, endoplasmic
reticulum and nuclear envelope. Bcl-2 is encoded by a 230 kb gene that
results in a 24-26 kDa protein. Bakhshi first identified Bcl-2 as an
apoptotic proto-oncogene while studying chromosomal translocations in
human follicular lymphomas. At approximately the same time Tsujimoto and
Croce made the same observation. The function of Bcl-2 was first
described by Vaux when it was demonstrated that it promoted the survival
of factor dependent cells. Bcl-2 overexpression has been implicated in a
wide variety of malignancies including (but not limited to) breast,
prostate, skin, colon and pancreatic cancers. Related anti-apoptotic
proteins include Bcl-xL, Mcl-1 and Bcl-w. Pro-apoptotic proteins in the
Bcl-2 family include Bax, BAD, Bak, Blk and Bid.
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|
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet the yellow Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet the blue Conjugate into the wells and incubtate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop solution and read plate on a plate reader at 450nm.
9. Calculate sample concentrations from the Standard Curve.
|
| |
human total Bcl-2
(900-133) |
| RANGE |
18.8 - 1,200 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
39 in duplicate |
| SENSITIVITY |
3.8 pg/mL |
| PRECISION |
2.4 - 4.2% Intra
3.4 - 4.2% Inter |
SAMPLE TYPE
recommended dilution |
Cell Lysis Buffer 2 ( 1:40)
2 million Jurkat cells per mL (1:40)
|
| TIME TO ANSWER |
3 Hours |
| SPECIES
SPECIFICITY |
human |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| human Bcl-2 |
100% |
|
|
| <0.1%: Bad /
Bax / Bid / Bcl-XL / cIAP1 / total JNK / pTEN / Smac/DIABLO /
beta-catenin |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45