FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Stable Color-Coded Liquid Reagents
Arginine Vasopressin (AVP) is a nonopeptide hormone and neurotransmitter
synthesized in hypothalamic supraoptic and paraventricular nuclei and
stored in the posterior pituitary. Also known as Antidiuretic Hormone (ADH),
AVP exerts powerful antidiuretic action on the kidney by increasing
permeability of the collecting tubules to water and urea. Osmotic and
non-osmotic stimuli prompt AVP release into peripheral circulation where
it affects a variety of anatomical and physiological conditions
including emotional stress, posture, blood volume and temperature
functions. Alcohol appears to inhibit AVP secretion. Serum AVP
measurement is used clinically for studies involving diabetes insipidus,
syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production
and psychogenic water intoxication.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Conjugate followed by the yellow Antibody into the
wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.