FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Unsurpassed Purity and Consistency
High Activity
Assay Designs offers a range of chemiluminescent molecules that can be
used for a variety of detection applications. The stability of these
compounds makes them ideal materials for the development of measurement
systems where robustness is essential.
The two compounds shown, the 1- and 2-Anthryl Schiff's bases, are
actually adducts made during the synthesis of the Schiff's base from the
amine, 2 molecules of the ketone and oxygen. These adducts are
spontaneously formed upon synthesis of the base. In the presence of
strong base the Schiff's bases rearrange to form the anion of the 1,2
dioxetane. This dioxetane is very unstable and undergoes spontaneous
concerted decomposition to yield the formamido anion and ketone. The
emission of light is from the singlet excited state formed and gives
rise to yellow light at approximately 540 nm. The chemiluminescent
quantum yield of the 2-Anthryl derivative is reported to be 48%. The
mechanism is shown below:
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
