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17beta-Estradiol Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-008 |
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| 5 x 96 Well Kit |
901-008 |
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17beta-Estradiol Chemiluminescent (CLIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 1 X 96 Well Kit |
910-008 |
|
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|
|
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Two Kit Formats
Stable, Color-Coded Liquid Reagents
|
17beta-Estradiol is a steroid hormone produced mainly in the ovaries, by
the placenta during pregnancy, and to a lesser extent in the adrenal
cortices, testes and peripheral tissues. The hormone is synthesized
enzymatically from acetate, cholesterol, progesterone and testosterone.
In addition to the well-described anatomic and physiological regulation
of reproduction and secondary sex characteristics, it also influences
diverse activities such as bone growth, brain development and maturation
and the intracellular concentrations of calcium and certain second
messenger molecules.
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1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. EIA: Pipet Stop Solution and read on a plate reader at 405 nm.
CLIA: Read in a suitable luminometer or luminescence detector.
6. Calculate sample concentrations from Standard Curve.
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| |
17beta-Estradiol
EIA
(900-008 & 901-008) |
17beta-Estradiol
CLIA
(910-008) |
| RANGE |
29.3-30,000 pg/mL
|
14.6-15,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
50 µL |
| SAMPLES PER 96
WELL KIT |
38 in duplicate
|
38 in duplicate
|
| SENSITIVITY |
28.5 pg/mL
|
14.2 pg/mL
|
| 50% INTERCEPT |
956 pg/mL
|
292 pg/mL
|
| PRECISION |
8.1-9.2% Intra
5.2-7.4% Inter |
6.2-9.7% Intra
5.6-8.0% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
saliva ( 1:16)
Not Recommended for Serum and Plasma Samples
|
culture media (none)
saliva (1:100)
Not Recommended for Serum and Plasma Samples
|
| TIME TO ANSWER |
3 Hours |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| 17beta-Estradiol |
100% |
Estrone |
4.64% |
| Estriol |
0.53% |
Estrone-3-Sulfate |
0.36% |
| 17alpha-ethynylestradiol |
<0.2% |
Progesterone |
0.06% |
| Testosterone |
0.02% |
19-hydroxytestosterone |
<0.02% |
| Dehydroisoandrosterone |
<0.001% |
Dehydroisoandrosterone-3-sulfate |
<0.001% |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45