FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Stable, Color-Coded Liquid Reagents
The progestin 17 alpha Hydroxyprogesterone (17 alpha
Hydroxypregn-4-ene-3,20-dione; 17-OHP) is the product of steroid alpha
hydroxylation of
Progesterone by steroid 17 alpha-hydroxylase. 17 alpha
Hydroxyprogesterone can be converted into 11-deoxycortisol (by
hydroxylation at C21) or Androstenedione and acetate (by
cleavage of steroid C17 - C20 bond). Serum levels
of 17 alpha Hydroxyprogesterone have primarily been used in the clinical
setting as a means of diagnosis for 21-hyrdroxylase deficiency, a
congenital adrenal hyperplasia.
17 alpha hydroxyprogesterone
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:2)