FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Low Cross Reactivity to Other Eicosanoids
Time to Answer: Overnight
15-(S)-hydroxyeicosateraenoic acid (15(S)-HETE) is a hydroxy derivative
of arachidonic acid made by 15-lipoxygenase contained in blood platelets,
neutrophils, eosinophils and reticulocytes. It is the most abundent
eicosanoid in human bronchi. It has been shown to be chemotactic and
chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle
cells. 15(S)-HETE acts as a second messenger in angiotensin-II induced
aldosterone production. Evidence also suggests many other biological
activites for this compound including stimulating insulin secretion by
pancreatic tissue, inducing hyperphosphorylation of several major
cytoskeletal proteins, enhancing surface expression of integrin
receptors and platelet aggregation in tumor cells. It induces
immunosuppresion and inhibits ACTH corticosterone production.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
15(S)-HETE (900-051
& 901-051)
RANGE
78.1-20,000 pg/mL
SAMPLE SIZE
100 µL
SAMPLES PER 96
WELL KIT
39 in duplicate
SENSITIVITY
69.21 pg/mL
50% INTERCEPT
710 pg/mL
PRECISION
3.4-15.8% Intra
6.1-19.1% Inter
SAMPLE TYPE
recommended dilution
culture media (none)
urine (1:8)
EDTA plasma (1:4-32)
serum (1:16)
TIME TO ANSWER
Overnight
SPECIES
SPECIFICITY
non-specific
Compound
Cross Reactivity
Compound
Cross Reactivity
15(S)-HETE
100%
8,15-diHETE
1.00%
5,15-diHETE
1.00%
13(S)-HODE
0.60%
5-HETE
0.10%
PGD2
0.10%
PGF2alpha
0.10%
PGB2
0.10%
12(S)-HETE
<0.05%
Arachidonic Acid
<0.05%
PGE2
<0.05%
12(R)-HETE
<0.05%
Linoleic Acid
<0.05%
For more information :: Bioxys and Gentaur
BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:8)