| Correlate™-EIA (ELISA Kits) |
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 |
|
11-dehydro-TXB2 Colorimetric (EIA)
Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-092 |
|
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|
| 5 x 96 Well Kit |
901-092 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
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Small Sample Volume: 100 µL
Time to Answer: 3 Hours
|
11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a
major metabolite of
Thromboxane B2 (TXB2) found in urine and
plasma. It is produced by the dehydrogenation of the alcohol group on
the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase.
The detection of 11-dehydro-TXB2 is often used to measure
thromboxane production in vivo. Monitoring levels of this
metabolite is a helpful tool in the study of several diseases, such as
liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus
erythematosus, thrombosis diseases, and other diseases involving
platelet activation. 11-dehydro-TXB2 has also been used in
diabetes and asthma studies.
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1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
|
| |
11-dTXB2
(900-092 & 901-092) |
| RANGE |
9.8-10,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
38 in duplicate
|
| SENSITIVITY |
4.31 pg/mL
|
| 50% INTERCEPT |
162 pg/mL
|
| PRECISION |
8.1-15.3% Intra
12.6-17.5% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
urine (none)
|
| TIME TO ANSWER |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| 11-dehydro TXB2 |
100% |
PGE2 |
1.85% |
| TXB2 |
0.40% |
PGF2alpha |
0.20% |
| 6-keto-PGF1alpha |
0.17% |
PGD2 |
0.10% |
| 2,3-dinor TXB2 |
0.10% |
8-iso-PGF2alpha |
0.07% |
| 9alpha,11beta-PGF2alpha |
0.05% |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45