Features
Innovative
AquaPlasmid
represents a
breakthrough in plasmid DNA isolation. It combines the functions
of cell lysis, plasmid DNA extraction, and debris removal in a
single aqueous solution.
Simple
Add
AquaPlasmid to lyse the cells, extract the DNA, and precipitate
the cell debris. And then add isopropanol to precipitate pure
plasmid DNA from the cleared lysate.
Easy
No columns, no
resins, no slurries, no beads, no filters, no membranes, no
needles, no cartridges, no manifolds, no HPLC, no ultracentrifuge.
Nontoxic
No phenol, no
chloroform, no saturated butanol, no ethidium bromide, no cesium
chloride, no guanidine hydrochloride, no sodium hydroxide, no
buffer 1, 2, 3, 4, ..., or A, B, C, D, ...
Fast
One minute to
pellet the bacteria, five minutes to pellet bacterial debris, two
minutes to pellet plasmid DNA. From culture to DNA in 20-30
minutes for mini, midi, or maxiprep.
Pure
A260/A280 =
1.8-2.0. Plasmid DNA is free of enzyme inhibitors and excellent
for all molecular biology applications.
Scalable
One single
AquaPlasmid Kit does all: mini, midi, and maxi; no need to
purchase separate mini, midi, and maxi kits.
High
Yield
20, 200, 2000
ug of DNA can be obtained from 1 (mini), 10 (midi), 100 (maxi)
ml of overnight Terrific Broth cultures, which are close to the
yields obtained by other midi, maxi, mega kits.
Economic
AquaPlasmid
is priced at $199/kit for 300 minipreps, that is $0.66/miniprep
or approximately half the price of other plasmid DNA isolation
kits.
Comparison
Below is a
comparison of AquaPlasmid and a leading competitor. You can
easily see the advantages and benefits of using AquaPlasmid.
|
|
Competitor
Q |
AquaPlasmid |
|
Isolation Mechanism |
Selective Binding |
Selective Extraction |
|
Isolation Format |
Solid Support |
Aqueous
Solution |
|
Price ($/miniprep) |
1.16 |
0.66 |
|
Alkaline Lysis |
Yes |
No |
|
Guanidine HCl |
Yes |
No |
|
Scalable |
No |
Yes |
|
# of Solutions in the Kit |
7 |
1 |
|
Isolation Time (min) |
30 (mini)
120 (midi)
180 (maxi) |
30 (mini)
30 (midi)
30 (maxi) |
|
Yield (ug DNA/ml culture) |
1-3 (LB)
Can't use TB |
3-5
(LB)
10-20 (TB) |
|
Purity (A260/A280) |
1.8-2.0 |
1.8-2.0 |
|
Sequencing (Phred20) |
~600 |
~900 |
Click
HERE to view a typical sequencing chromatogram from
AquaPlasmid isolated DNA. Sequencing was done on an ABI3730
96-Capillary Sequencer using 4ul plasmid miniprep (100ul at 250
ng/ul).
Product Name:
AquaPlasmidTM
Kit
Product Number: 1000
Application:
Isolation of plasmid DNA. For
in vitro research use only.
Size:
The kit is sufficient for the preparation of
-
300
minipreps (1-3 ml culture for ~20 ug DNA)
-
or
30 midipreps (10-30 ml culture for ~200 ug DNA)
-
or 3
maxipreps (100-300 ml culture for ~2000 ug DNA)
Kit
Contents: The
AquaPlasmid Kit includes the following items
-
30
ml of AquaPlasmid Solution
-
Instruction Manual
Price:
€ 250 per kit
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45