| Retroviral vectors have
proven to be useful tools for either in vitro or in vivo
stable integration of a transduced gene into the host
cell chromosome via the integration mechanisms that they
normally employ during their normal life cycle.
One of the significant advances in retroviral
expression systems in recent years is the production of
high titer recombinant retrovirus by transient
transfection. The improvement allows for the production
of high titer retroviruses within a week and eliminates
the time-consuming process of generating a viral
producer cell line.
Our Retro-Easy™ retroviral
expression system includes a comprehensive line of
retroviral vectors with very flexible multiple cloning
sites for easy subcloning of your gene of interest into
any of our retroviral vectors. The His and HA fusion tag
compatible vectors (pRetro-E1™-HA and pRetro-E2™-His)
allows for fusion gene expression in retroviral vectors
with either a His or HA tag.
Retro-Easy™ Expression Systems
|
Product |
Description |
Unit
|
Cat. No
|
|
Retro-Easy1 |
Complete retroviral
expression system:
- pRetro-E1
plasmid
- Pack-Easy Cells
- pRetro-Combo Mix (5 rxns) |
Kit
|
E-512
|
|
Retro-Easy2 |
Complete retroviral expression system:
-
pRetro-E2 plasmid
- Pack-Easy Cells
- pRetro-Combo Mix (5 rxns) |
Kit
|
E-513
|
|
Retro-Easy-HA |
Complete retroviral expression system:
- pRetro-E1-HA
plasmid
- Pack-Easy Cells
- pRetro-Combo Mix (5 rxns) |
Kit
|
E-518
|
|
Retro-Easy-His |
Complete retroviral expression system:
- pRetro-E2-His
plasmid
- Pack-Easy Cells
- pRetro-Combo Mix (5 rxns) |
Kit
|
E-519
|
Retro-Easy™ Vectors
|
Product |
Description |
Qty
|
Cat. No
|
|
pRetro-E1 |
Retroviral vector with
Neomycine selection marker |
10 µg
|
E-514
|
|
pRetro-E2 |
Retroviral vector with Puromycine selection
marker |
10 µg
|
E-515
|
|
pRetro-E1-HA |
Retroviral vector with HA tag |
10 µg
|
E-516
|
|
pRetro-E2-His |
Retroviral vector with His tag |
10 µg
|
E-517
|
Related Products
|
Product |
Description |
Qty
|
Cat. No
|
| Retro-Combo Mix |
Retrovirus packaging
plasmid mix |
10 rxns
|
E-510
|
| Pack-Easy Cells |
Retrovirus packaging cell lines |
vial
|
E-511
|
| EGFP Control |
EGFP Retrovirus |
20 ml
|
C014
|
| β-Gal Control |
β-Gal Retrovirus |
20 ml
|
C033
|
|
Anti-HA |
Tag Monoclonal Antibody
|
100ug
|
G036
|
|
Anti-His |
Tag Monoclonal Antibody |
100ug
|
G020
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45