| |
Description:
The Best Transfection Reagent. Applicable to cells that
are difficult to transfect. Celfectin™ is specially formulated
with multiple cationic polymers.Application:
Transfection of DNA into cultured eukaryotic cells.
Recommended storage conditions:
Store at 4ºC. Do not freeze.
Protocol:
|
Table 1. Celfectin™ achieves higher
transfection efficiency than Lipofectamine™ and
Lipofectamine™ 2000 in the following cell lines.
|
|
Cell Line
|
% transfection efficiency
|
|
Celfectin™
|
Lipofectamine
|
Lipofectamine 2000
|
| SKOV3 |
15%
|
n/a
|
0%
|
| HEY (Ovarian carcinoma) |
80-90%
|
10-15%
|
n/a
|
| Vero |
10%
|
<1%
|
n/a
|
| MMRU melanoma |
50%
|
n/a
|
5%
|
Table 2. Celfectin™ has been tested in the
following cell lines.
| Vero, African green
monkey kidney |
PC3, human prostate
carcinoma |
H460, human pleural
effusion adenocarcinoma |
| 293, human kidney |
SF188, human glioma |
COS7, monkey kidney |
| U87, human glioma |
Hela, human crevix carcinoma |
3T3, mouse embryonic fibroblasts |
| MCF7, human breast adenocarcinoma |
HepG2, human hepatoma |
LNcaP, human prostate carcinoma |
| SKOV3 |
HEY, ovarian carcinoma |
Caco-2, human colorectal
adenocarcinoma |
| Jurkat, human T cell leukemia |
B16, mouse melanoma |
MMRU melanoma |
.
This product is distributed
for laboratory research only.
Caution: Not for diagnostic use . |
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