Anti-NF-L Chicken PA

Anti-NF-L Chicken Polyclonal Antibody

	Chicken Polyclonal
	Catalog #: CA-19

	Quantity: 100μl
	Clone: CPCA-NF-L

	Immunogen: To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.

	Specificity: NF-L from human, rat, mouse, cat, chicken

	Formulation: 1mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid

	Storage/Stability: Stable for one year at –20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.

	Application: The IgY solution can be used at dilutions of at least 1:2,500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5,000 or lower.


	This product is distributed for laboratory research only. Caution: Not for diagnostic use

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