Anti-NF-H Chicken PA

Anti-NF-H Chicken Polyclonal Antibody

	Chicken Polyclonal
	Catalog #: CA-17

	Quantity: 100μl
	Clone: CPCA-NFH

	Immunogen: To raise this antibody bovine intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell.

	Specificity: NF-H from human, rat, mouse, cat, chicken

	Formulation: 1mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid.

	Storage/Stability: Stable for one year at –20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.

	Application: The IgY solution has an extremely high titre and can be used at dilutions of at least 1:50,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:500,000 or lower. The antibody has also been used successfully in the capture role in ELISA.


	Reference: Biochem Biophys Res Commun. 336:1268-1277 (2005).


	This product is distributed for laboratory research only. Caution: Not for diagnostic use

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