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Chicken Polyclonal
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Catalog #: CA-27 |
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Quantity: 100μl |
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Clone: CPCA-a-Int |
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Immunogen: Recombinant
a-internexin expressed in and purified from E. coli. |
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Specificity: Alpha-internexin (a.k.a.
NF66) in human, rat, mouse, cat, and other mammals. |
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Formulation: 1mg/ml in PBS, pH
7.4 with 0.05% sodium azide. Frozen liquid. |
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Storage/Stability: Stable for
one year at –20ºC from date of shipment. For maximum recovery of
product, centrifuge the original vial after thawing and prior to
removing the cap. Aliquot to avoid repeated freezing and thawing.
Aliquot will be stable at 4ºC for 3 months. |
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Application: The serum can be
diluted to 1:500 and higher for immunofluorescence staining and
1:5,000 and higher for western blotting. On western blots use a
brain or spinal cord homogenate and look for a major band at
SDS-PAGE apparent molecular weight of 66 to 68kDa, depending on
the species. The human protein runs at ~68kDa, while rat, mouse
and other species run at ~66kDa. Minor bands at ~150kDa are
probably covalent dimers, and bands at ~50kDa represent
a-internexin breakdown products.
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Reference:
J Cell Biol 101:1316-22 (1985)
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This product is
distributed for laboratory research only.
Caution: Not for diagnostic use |
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