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Mouse Monoclonal
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Catalog #: YA38F3 |
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Quantity: 100μl |
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Clone: 38F3 |
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Immunogen: Raised against yeast
fibrillarin, a highly conserved nucleolar protein present in
small nucleolar ribonucleoprotein (snoRNP) complexes. |
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Specificity: Cross reacts with
fibrillarin in human, rat, plant, Drosophila, C. elegans and S.
pombe. |
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Formulation: 1mg/ml in PBS, pH
7.4 with 0.05% sodium azide. Frozen liquid. |
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Storage/Stability: Stable for 1
year at -20°C from date of shipment. For maximum recovery of
product, centrifuge the original vial after thawing and prior to
removing the cap. Aliquot to avoid repeated freezing and thawing.
Aliquot will be stable at 4°C for 3 months. |
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Application: For western blots
of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear
fractions), followed by chemiluminescent detection (ECL). For
other (non-ECL) western detection methods: 1:500 to 1:2,500. To
detect mammalian fibrillarin on western blots by ECL: 1:250. For
immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of
mammalian cells: 1:250.
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Reference:
Biol Cell 54:123-133 (1985).
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This product is
distributed for laboratory research only.
Caution: Not for diagnostic use |
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